How NAD+ Testing Works

Precise biomarker testing from home - understand your cellular health with laboratory-grade accuracy

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Sample Collection

Your test kit arrives within 3-5 business days with everything needed for accurate sample collection. Using a simple finger-prick method, you'll collect a small dried blood spot sample on our specialized filter paper. The process takes just a few minutes and can be completed entirely at home.

Your kit includes:

  • Dried blood spot collection card
  • Sterile lancets for safe sample collection
  • Alcohol prep pads and gauze
  • Capillary collection tube with precise measurement markers
  • Protective storage pouch with desiccant pack
  • Prepaid shipping materials for laboratory transport
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Laboratory Analysis

Once your sample reaches our certified laboratory, we use advanced bioluminescent detection technology to measure your NAD+ levels. Our analysis method employs modified luciferin proteins that bind specifically to NAD+ molecules and emit measurable light signals proportional to your NAD+ concentration.

The testing process: Your dried blood sample is processed using our proprietary NAD+ detection assay. Modified jellyfish proteins (luciferins) are introduced to your sample, where they bind exclusively to NAD+ molecules. The resulting bioluminescent signal is measured using precision photometry equipment, providing quantitative NAD+ level data.

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Results & Analysis

Within 1-2 weeks, you'll receive your comprehensive NAD+ report through our secure online portal. Your results include your measured NAD+ levels, reference ranges, and personalized insights based on your biomarker data.

  • How the NAD+ Detection Technology Works

    Bioluminescent biomarker detection – the science behind your results

  • Our NAD+ testing uses a breakthrough detection method based on modified bioluminescent proteins originally found in jellyfish. Here's how we measure your NAD+ levels with laboratory precision:

  • Protein Binding

    Modified luciferin proteins are introduced to your blood sample, where they bind exclusively to NAD+ molecules

  • Light Emission

    When bound to NAD+, these proteins emit a bioluminescent signal

  • Quantification

    The intensity of the light signal directly correlates to the amount of NAD+ present in your sample

  • Analysis

    Precision photometry equipment measures the bioluminescent output to determine your exact NAD+ concentration

Testing Timeline & Process

Days 1-5

Kit delivery to your address

Day of collection

5-minute sample collection and same-day shipping to lab

3-5 Days Later

Laboratory analysis and report generation

Results delivery

Secure online access to your NAD+ biomarker data

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Sample Collection Protocol

Important timing

Ensure you can ship your sample to our laboratory within 24 hours of collection using the included prepaid UPS shipping materials.

For supplement tracking

If monitoring NAD+ response to precursors, we recommend baseline testing after a 2-week washout period, followed by testing after 30 days of consistent supplementation protocol.

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Collection Procedure

Preparation: Complete the sample collection card with your personal information and collection date. Wash hands thoroughly with warm water to promote circulation.

  • Sterilize fingertip (ring or middle finger side) with provided alcohol pad
  • Use sterile lancet to create small puncture wound
  • Allow blood droplet to form naturally
  • Fill capillary tube to the designated measurement line
  • Transfer blood to filter paper using the tube's plunger mechanism
  • Allow sample to dry completely (minimum 3 hours)

Sample Preservation: Seal dried blood spot card with desiccant pack in the protective pouch. Ship within 24 hours using provided materials to ensure sample integrity.

  • Dr. Rebecca Crews

    Research Director

    Renue By Science
    • Helped develop the NAD+ blood testing protocol
    • Expert in nutritional NAD+ blood testing protocol
  • Dr. Qiuliyang Yu

    Principal Investigator

    Shenzhen Institute
    • Engineered the NAD+ testing biosensor
    • Expert in nutritional NAD+ blood testing protocol
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Bioluminescent Detection Technology

Our testing method harnesses modified luciferin proteins derived from bioluminescent jellyfish. These engineered proteins have been specifically designed to bind exclusively to NAD+ molecules. Upon binding, they emit quantifiable light signals that allow precise measurement of NAD+ concentrations in biological samples.

The intensity of bioluminescent output directly correlates with NAD+ levels, providing accurate biomarker data comparable to traditional laboratory methods while enabling convenient at-home sample collection.

Understanding Your NAD+ Levels

NAD+ levels serve as a key indicator of cellular health and metabolic function. As we age, NAD+ naturally declines, beginning in our 30s, which correlates with reduced cellular energy production and repair capacity.

Factors influencing NAD+ levels

  • Sleep quality and duration
  • Exercise frequency and intensity
  • Stress management
  • Nutritional choices
  • NAD+ precursor supplementation (NMN, NR, Trigonelline)

Individual variation in NAD+ response

Research shows significant individual differences in how people respond to NAD+ precursor supplementation. Some individuals show marked increases in NAD+ levels, while others show minimal response to the same protocols. Testing allows you to determine your personal response to interventions.

How measurement matters

NAD+ testing removes guesswork from your health optimization strategy. By establishing your baseline levels and monitoring changes over time, you can make data-driven decisions about lifestyle modifications and supplementation protocols.

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Ready to Measure Your NAD+ Levels?

Understanding your NAD+ biomarker provides concrete data about your cellular health status. Whether you're evaluating the effectiveness of NAD+ precursor supplementation or assessing how lifestyle factors impact your cellular function, testing gives you the information needed to make informed health decisions.