How NAD+ Testing Works
Precise biomarker testing from home - understand your cellular health with laboratory-grade accuracy

1
Sample Collection
Your test kit arrives within 3-5 business days with everything needed for accurate sample collection. Using a simple finger-prick method, you'll collect a small dried blood spot sample on our specialized filter paper. The process takes just a few minutes and can be completed entirely at home.
Your kit includes:
- Dried blood spot collection card
- Sterile lancets for safe sample collection
- Alcohol prep pads and gauze
- Capillary collection tube with precise measurement markers
- Protective storage pouch with desiccant pack
- Prepaid shipping materials for laboratory transport

2
Laboratory Analysis
Once your sample reaches our certified laboratory, we use advanced bioluminescent detection technology to measure your NAD+ levels. Our analysis method employs modified luciferin proteins that bind specifically to NAD+ molecules and emit measurable light signals proportional to your NAD+ concentration.
The testing process: Your dried blood sample is processed using our proprietary NAD+ detection assay. Modified jellyfish proteins (luciferins) are introduced to your sample, where they bind exclusively to NAD+ molecules. The resulting bioluminescent signal is measured using precision photometry equipment, providing quantitative NAD+ level data.

3
Results & Analysis
Within 1-2 weeks, you'll receive your comprehensive NAD+ report through our secure online portal. Your results include your measured NAD+ levels, reference ranges, and personalized insights based on your biomarker data.
Testing Timeline & Process
Days 1-5
Kit delivery to your address
Day of collection
5-minute sample collection and same-day shipping to lab
3-5 Days Later
Laboratory analysis and report generation
Results delivery
Secure online access to your NAD+ biomarker data

Sample Collection Protocol
Important timing
Ensure you can ship your sample to our laboratory within 24 hours of collection using the included prepaid UPS shipping materials.
For supplement tracking
If monitoring NAD+ response to precursors, we recommend baseline testing after a 2-week washout period, followed by testing after 30 days of consistent supplementation protocol.

Collection Procedure
Preparation: Complete the sample collection card with your personal information and collection date. Wash hands thoroughly with warm water to promote circulation.
- Sterilize fingertip (ring or middle finger side) with provided alcohol pad
- Use sterile lancet to create small puncture wound
- Allow blood droplet to form naturally
- Fill capillary tube to the designated measurement line
- Transfer blood to filter paper using the tube's plunger mechanism
- Allow sample to dry completely (minimum 3 hours)
Sample Preservation: Seal dried blood spot card with desiccant pack in the protective pouch. Ship within 24 hours using provided materials to ensure sample integrity.

Bioluminescent Detection Technology
Our testing method harnesses modified luciferin proteins derived from bioluminescent jellyfish. These engineered proteins have been specifically designed to bind exclusively to NAD+ molecules. Upon binding, they emit quantifiable light signals that allow precise measurement of NAD+ concentrations in biological samples.
The intensity of bioluminescent output directly correlates with NAD+ levels, providing accurate biomarker data comparable to traditional laboratory methods while enabling convenient at-home sample collection.
Understanding Your NAD+ Levels
NAD+ levels serve as a key indicator of cellular health and metabolic function. As we age, NAD+ naturally declines, beginning in our 30s, which correlates with reduced cellular energy production and repair capacity.
Factors influencing NAD+ levels
- Sleep quality and duration
- Exercise frequency and intensity
- Stress management
- Nutritional choices
- NAD+ precursor supplementation (NMN, NR, Trigonelline)
Individual variation in NAD+ response
Research shows significant individual differences in how people respond to NAD+ precursor supplementation. Some individuals show marked increases in NAD+ levels, while others show minimal response to the same protocols. Testing allows you to determine your personal response to interventions.
How measurement matters
NAD+ testing removes guesswork from your health optimization strategy. By establishing your baseline levels and monitoring changes over time, you can make data-driven decisions about lifestyle modifications and supplementation protocols.

Ready to Measure Your NAD+ Levels?
Understanding your NAD+ biomarker provides concrete data about your cellular health status. Whether you're evaluating the effectiveness of NAD+ precursor supplementation or assessing how lifestyle factors impact your cellular function, testing gives you the information needed to make informed health decisions.